anti-[SW|sigma factor] to [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF], protein serine kinase, phosphorylates [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA]
function
control of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activity; phosphorylation and inactivation of [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA]
product
anti-[SW|sigma factor], protein serine kinase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors][category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]Gene
Coordinates
2,444,208 → 2,444,648
The protein
Catalyzed reaction/ biological activity
phosphorylation of [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA]negative regulator (by binding) of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]Protein family
anti-sigma-factor family (with [protein|5AF5F199C5D92E13DE1C56E28948A557E3954CBF|RsbW], according to UniProt)Structure
[PDB|1L0O] (complex with [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF], Geobacillus stearothermophilus) [pubmed|11955433][PDB|1TIL] (complex with [protein|3936E91C062074BFE4284B54A8CC33F35F94F5EE|SpoIIAA], Geobacillus stearothermophilus) [pubmed|15236958]Expression and Regulation
Operons
genes
[gene|3936E91C062074BFE4284B54A8CC33F35F94F5EE|spoIIAA]-[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]-[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]
description
[Pubmed|22383849]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon][protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon][protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: activation, [PubMed|1556084,15687200], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon][protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon][protein|5A6FBAE6553343092862CB79E150F934978C32A9|SinR]: repression, in [regulon|5A6FBAE6553343092862CB79E150F934978C32A9|SinR regulon]regulation
''[SW|spoIIAA]'': expressed early during sporulationview in new tabgenes
[gene|F7AD78F9AB98A150E8CDFC1D01A9F938FF9F8BD1|dacF]-[gene|3936E91C062074BFE4284B54A8CC33F35F94F5EE|spoIIAA]-[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]-[gene|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|sigF]
description
[Pubmed|22383849]
sigma factors
[protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|15699190], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon][protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]regulatory mechanism
[protein|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB]: repression, [Pubmed|20817675], in [regulon|E5110D9C36E8C55AFD1419987B14A53232165F20|AbrB regulon]regulation
''[SW|spoIIAA]'': expressed early during sporulationstrongly repressed in the presence of salt (1.2 M NaCl) [pubmed|32419322]view in new tabBiological materials
Mutant
BKE23460 (Δ[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAACTCAAGGTGCATTTCAT, downstream forward: _UP4_CTTTGTAATTAAGGAGATTTBKK23460 (Δ[gene|C8B867758330355F44C63CC284DC7AA3061FB9F1|spoIIAB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_AAACTCAAGGTGCATTTCAT, downstream forward: _UP4_CTTTGTAATTAAGGAGATTTlabs
[SW|Charles Moran], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage]References
Reviews
Modeling of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activation
24067622,22312331,20298743 Original Publications
8358793,7570023,15023063,15351644,8460142,9077448,12923101,8820658,10864495,16824103,12867473,8955289,10476035,8764398,8764397,14744853,9826498,9826499,15175314,1938874,12676949,8126438,11684022,8846916,1948031,3114419,11846550,15201047,2123551,15205443,8885263,8332059,8168129,11701124,8824593,1556084,15687200,20817675,25278935,11955433,15236958